draw ultra-version 14.0 software Search Results


chembiodraw ultra version 14.0.0.117 software naming program  (CambridgeSoft Corporation)
90
CambridgeSoft Corporation chembiodraw ultra version 14.0.0.117 software naming program
Chembiodraw Ultra Version 14.0.0.117 Software Naming Program, supplied by CambridgeSoft Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/draw+ultra-version+14%2E0+software/us11274093-322-26-33?v=CambridgeSoft+Corporation
Average 90 stars, based on 1 article reviews
chembiodraw ultra version 14.0.0.117 software naming program - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
femtosecond laser  (Coherent Corp)
96
Coherent Corp femtosecond laser
(A) A schematic illustration of 2P3A-DSLM. We used a commercial Ti-sapphire <t>femtosecond</t> laser (Chameleon Version II, Coherent) as the illumination light source, and expanded the beam by a pair of concave mirrors. The focal length of the TAG lens was modulated sinusoidally at frequency of 100-500 kHz (diopter, ±30), which rapidly scanned the laser focus along the optics axis ( x -direction) in front of the illumination objective (IO, 40× NA 0.8, Nikon). The GSM scanned the laser beam vertically to the illumination objective ( y -direction). The TAG and the GSM were positioned at two conjugate planes of the back focal plane (BFP) of the illumination objective by L1 (50 mm), L2 (100 mm), L3 (100 mm) and L4 (200 mm). This configuration minimized the distortion and divergence of illumination intensity during scanning. The detection objective (DO, 40× NA 0.8, Nikon), emission filter, tube lens (200 mm) and sCMOS camera (flash 4.0, 2 048 × 2 048, Hamamatsu) were carefully aligned to record fluorescence images. A USB-controlled 3D stage (Thorlabs, MT3-Z8) combined with a fast Piezo Z-stepper (Edmund, Nanopositioning Piezo) was used to translate the sample for fast 3D imaging. The structure of a TAG len is given in the inset , . EF, emission filter; TL, tunable lens. (B - D) Tailorable light sheet size from less than 10 × 10 μm 2 to 170 × 170 μm 2 driven by intensity modulation of the TAG. (E) The DOF was linearly related with the increase of TAG modulation intensity. (F) The thickness of the light sheet at the near-end and the middle remained nearly constant below 1 μm, while the thickness at the far-end increased to 1.5 μm when DOF reached 170 μm. (G) The axial resolution is below 1 μm under all circumstances except at the far-end of the 170 μm DOF (TAG modulation, 35%). (H - I) Dynamic imaging of mitochondria in live mt-cpYFP transgenic C. elegans pharynx on adult day 3 (also refer to ) revealed fast split in the mitoflash fluorescence trace. (J - L) 2P3A-DSLM 3D imaging of DAPI-labeled (2 μg/ml) nuclei in the heart of a live zebrafish. (J) A selected section from the Z-stack movie ; (K) Selected area in J shows cells in division phase and inter-phase. Dividing chromosomes within single nucleus were clearly resolved; (L) The fluorescence profile of the red line imposed on the chromosome shown in K . Images in H , J and K were deconvolved using ImageJ RL algorithm. Scale bar: 100 μm (B - D) , 20 μm (H) , 50 μm (J) and 5 μm (K) .
Femtosecond Laser, supplied by Coherent Corp, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/draw+ultra-version+14%2E0+software/pmc04650563-11-14-25?v=Coherent+Corp
Average 96 stars, based on 1 article reviews
femtosecond laser - by Bioz Stars, 2026-08
96/100 stars
  Buy from Supplier
chembio3d ultra  (CambridgeSoft Corporation)
90
CambridgeSoft Corporation chembio3d ultra
(A) A schematic illustration of 2P3A-DSLM. We used a commercial Ti-sapphire <t>femtosecond</t> laser (Chameleon Version II, Coherent) as the illumination light source, and expanded the beam by a pair of concave mirrors. The focal length of the TAG lens was modulated sinusoidally at frequency of 100-500 kHz (diopter, ±30), which rapidly scanned the laser focus along the optics axis ( x -direction) in front of the illumination objective (IO, 40× NA 0.8, Nikon). The GSM scanned the laser beam vertically to the illumination objective ( y -direction). The TAG and the GSM were positioned at two conjugate planes of the back focal plane (BFP) of the illumination objective by L1 (50 mm), L2 (100 mm), L3 (100 mm) and L4 (200 mm). This configuration minimized the distortion and divergence of illumination intensity during scanning. The detection objective (DO, 40× NA 0.8, Nikon), emission filter, tube lens (200 mm) and sCMOS camera (flash 4.0, 2 048 × 2 048, Hamamatsu) were carefully aligned to record fluorescence images. A USB-controlled 3D stage (Thorlabs, MT3-Z8) combined with a fast Piezo Z-stepper (Edmund, Nanopositioning Piezo) was used to translate the sample for fast 3D imaging. The structure of a TAG len is given in the inset , . EF, emission filter; TL, tunable lens. (B - D) Tailorable light sheet size from less than 10 × 10 μm 2 to 170 × 170 μm 2 driven by intensity modulation of the TAG. (E) The DOF was linearly related with the increase of TAG modulation intensity. (F) The thickness of the light sheet at the near-end and the middle remained nearly constant below 1 μm, while the thickness at the far-end increased to 1.5 μm when DOF reached 170 μm. (G) The axial resolution is below 1 μm under all circumstances except at the far-end of the 170 μm DOF (TAG modulation, 35%). (H - I) Dynamic imaging of mitochondria in live mt-cpYFP transgenic C. elegans pharynx on adult day 3 (also refer to ) revealed fast split in the mitoflash fluorescence trace. (J - L) 2P3A-DSLM 3D imaging of DAPI-labeled (2 μg/ml) nuclei in the heart of a live zebrafish. (J) A selected section from the Z-stack movie ; (K) Selected area in J shows cells in division phase and inter-phase. Dividing chromosomes within single nucleus were clearly resolved; (L) The fluorescence profile of the red line imposed on the chromosome shown in K . Images in H , J and K were deconvolved using ImageJ RL algorithm. Scale bar: 100 μm (B - D) , 20 μm (H) , 50 μm (J) and 5 μm (K) .
Chembio3d Ultra, supplied by CambridgeSoft Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/draw+ultra-version+14%2E0+software/pmc06071921-229-18-22?v=CambridgeSoft+Corporation
Average 90 stars, based on 1 article reviews
chembio3d ultra - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
ultra version 14 0  (Chembio Diagnostics)
86
Chembio Diagnostics ultra version 14 0
(A) A schematic illustration of 2P3A-DSLM. We used a commercial Ti-sapphire <t>femtosecond</t> laser (Chameleon Version II, Coherent) as the illumination light source, and expanded the beam by a pair of concave mirrors. The focal length of the TAG lens was modulated sinusoidally at frequency of 100-500 kHz (diopter, ±30), which rapidly scanned the laser focus along the optics axis ( x -direction) in front of the illumination objective (IO, 40× NA 0.8, Nikon). The GSM scanned the laser beam vertically to the illumination objective ( y -direction). The TAG and the GSM were positioned at two conjugate planes of the back focal plane (BFP) of the illumination objective by L1 (50 mm), L2 (100 mm), L3 (100 mm) and L4 (200 mm). This configuration minimized the distortion and divergence of illumination intensity during scanning. The detection objective (DO, 40× NA 0.8, Nikon), emission filter, tube lens (200 mm) and sCMOS camera (flash 4.0, 2 048 × 2 048, Hamamatsu) were carefully aligned to record fluorescence images. A USB-controlled 3D stage (Thorlabs, MT3-Z8) combined with a fast Piezo Z-stepper (Edmund, Nanopositioning Piezo) was used to translate the sample for fast 3D imaging. The structure of a TAG len is given in the inset , . EF, emission filter; TL, tunable lens. (B - D) Tailorable light sheet size from less than 10 × 10 μm 2 to 170 × 170 μm 2 driven by intensity modulation of the TAG. (E) The DOF was linearly related with the increase of TAG modulation intensity. (F) The thickness of the light sheet at the near-end and the middle remained nearly constant below 1 μm, while the thickness at the far-end increased to 1.5 μm when DOF reached 170 μm. (G) The axial resolution is below 1 μm under all circumstances except at the far-end of the 170 μm DOF (TAG modulation, 35%). (H - I) Dynamic imaging of mitochondria in live mt-cpYFP transgenic C. elegans pharynx on adult day 3 (also refer to ) revealed fast split in the mitoflash fluorescence trace. (J - L) 2P3A-DSLM 3D imaging of DAPI-labeled (2 μg/ml) nuclei in the heart of a live zebrafish. (J) A selected section from the Z-stack movie ; (K) Selected area in J shows cells in division phase and inter-phase. Dividing chromosomes within single nucleus were clearly resolved; (L) The fluorescence profile of the red line imposed on the chromosome shown in K . Images in H , J and K were deconvolved using ImageJ RL algorithm. Scale bar: 100 μm (B - D) , 20 μm (H) , 50 μm (J) and 5 μm (K) .
Ultra Version 14 0, supplied by Chembio Diagnostics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/draw+ultra-version+14%2E0+software/pm41115483-70-8-6?v=Chembio+Diagnostics
Average 86 stars, based on 1 article reviews
ultra version 14 0 - by Bioz Stars, 2026-08
86/100 stars
  Buy from Supplier

Image Search Results


(A) A schematic illustration of 2P3A-DSLM. We used a commercial Ti-sapphire femtosecond laser (Chameleon Version II, Coherent) as the illumination light source, and expanded the beam by a pair of concave mirrors. The focal length of the TAG lens was modulated sinusoidally at frequency of 100-500 kHz (diopter, ±30), which rapidly scanned the laser focus along the optics axis ( x -direction) in front of the illumination objective (IO, 40× NA 0.8, Nikon). The GSM scanned the laser beam vertically to the illumination objective ( y -direction). The TAG and the GSM were positioned at two conjugate planes of the back focal plane (BFP) of the illumination objective by L1 (50 mm), L2 (100 mm), L3 (100 mm) and L4 (200 mm). This configuration minimized the distortion and divergence of illumination intensity during scanning. The detection objective (DO, 40× NA 0.8, Nikon), emission filter, tube lens (200 mm) and sCMOS camera (flash 4.0, 2 048 × 2 048, Hamamatsu) were carefully aligned to record fluorescence images. A USB-controlled 3D stage (Thorlabs, MT3-Z8) combined with a fast Piezo Z-stepper (Edmund, Nanopositioning Piezo) was used to translate the sample for fast 3D imaging. The structure of a TAG len is given in the inset , . EF, emission filter; TL, tunable lens. (B - D) Tailorable light sheet size from less than 10 × 10 μm 2 to 170 × 170 μm 2 driven by intensity modulation of the TAG. (E) The DOF was linearly related with the increase of TAG modulation intensity. (F) The thickness of the light sheet at the near-end and the middle remained nearly constant below 1 μm, while the thickness at the far-end increased to 1.5 μm when DOF reached 170 μm. (G) The axial resolution is below 1 μm under all circumstances except at the far-end of the 170 μm DOF (TAG modulation, 35%). (H - I) Dynamic imaging of mitochondria in live mt-cpYFP transgenic C. elegans pharynx on adult day 3 (also refer to ) revealed fast split in the mitoflash fluorescence trace. (J - L) 2P3A-DSLM 3D imaging of DAPI-labeled (2 μg/ml) nuclei in the heart of a live zebrafish. (J) A selected section from the Z-stack movie ; (K) Selected area in J shows cells in division phase and inter-phase. Dividing chromosomes within single nucleus were clearly resolved; (L) The fluorescence profile of the red line imposed on the chromosome shown in K . Images in H , J and K were deconvolved using ImageJ RL algorithm. Scale bar: 100 μm (B - D) , 20 μm (H) , 50 μm (J) and 5 μm (K) .

Journal: Cell Research

Article Title: Large-field high-resolution two-photon digital scanned light-sheet microscopy

doi: 10.1038/cr.2014.124

Figure Lengend Snippet: (A) A schematic illustration of 2P3A-DSLM. We used a commercial Ti-sapphire femtosecond laser (Chameleon Version II, Coherent) as the illumination light source, and expanded the beam by a pair of concave mirrors. The focal length of the TAG lens was modulated sinusoidally at frequency of 100-500 kHz (diopter, ±30), which rapidly scanned the laser focus along the optics axis ( x -direction) in front of the illumination objective (IO, 40× NA 0.8, Nikon). The GSM scanned the laser beam vertically to the illumination objective ( y -direction). The TAG and the GSM were positioned at two conjugate planes of the back focal plane (BFP) of the illumination objective by L1 (50 mm), L2 (100 mm), L3 (100 mm) and L4 (200 mm). This configuration minimized the distortion and divergence of illumination intensity during scanning. The detection objective (DO, 40× NA 0.8, Nikon), emission filter, tube lens (200 mm) and sCMOS camera (flash 4.0, 2 048 × 2 048, Hamamatsu) were carefully aligned to record fluorescence images. A USB-controlled 3D stage (Thorlabs, MT3-Z8) combined with a fast Piezo Z-stepper (Edmund, Nanopositioning Piezo) was used to translate the sample for fast 3D imaging. The structure of a TAG len is given in the inset , . EF, emission filter; TL, tunable lens. (B - D) Tailorable light sheet size from less than 10 × 10 μm 2 to 170 × 170 μm 2 driven by intensity modulation of the TAG. (E) The DOF was linearly related with the increase of TAG modulation intensity. (F) The thickness of the light sheet at the near-end and the middle remained nearly constant below 1 μm, while the thickness at the far-end increased to 1.5 μm when DOF reached 170 μm. (G) The axial resolution is below 1 μm under all circumstances except at the far-end of the 170 μm DOF (TAG modulation, 35%). (H - I) Dynamic imaging of mitochondria in live mt-cpYFP transgenic C. elegans pharynx on adult day 3 (also refer to ) revealed fast split in the mitoflash fluorescence trace. (J - L) 2P3A-DSLM 3D imaging of DAPI-labeled (2 μg/ml) nuclei in the heart of a live zebrafish. (J) A selected section from the Z-stack movie ; (K) Selected area in J shows cells in division phase and inter-phase. Dividing chromosomes within single nucleus were clearly resolved; (L) The fluorescence profile of the red line imposed on the chromosome shown in K . Images in H , J and K were deconvolved using ImageJ RL algorithm. Scale bar: 100 μm (B - D) , 20 μm (H) , 50 μm (J) and 5 μm (K) .

Article Snippet: Here we used the TAG lens to rapidly translate the focal spot of a femtosecond laser (140 fs, repetition rate, 80 MHz, Chameleon Version II, Coherent) along the optics axis ( x -direction) in front of the illumination objective (40× NA 0.8, Nikon).

Techniques: Fluorescence, Imaging, Transgenic Assay, Labeling